We have therefore established a whole blood assay that only requires 100 μL heparinized blood and utilizes the dihydrorhodamine DHR 123 ROS probe combined with cell surface antibody staining for the specific detection of ROS in several subsets of cells simultaneously using flow cytometry
The dihydrorhodamine 123 assay is generally applied to measure the production of intracellular reactive oxygen species in neutrophils using flow cytometry and is considered a diagnostic evaluation for chronic granulomatous disease
Chronic granulomatous disease Diagnosis and treatment
Dihydrorhodamine 123 an overview ScienceDirect Topics
Background Dihydrorhodamine DHR flow cytometric analysis is used to evaluate granulocyte oxidative bursts and is the test of choice for the diagnosis of chronic granulomatous disease CGD We present the clinical and DHR test profiles of five subjects assessed during and after acute illness
The dihydrorhodamine test can turn positive in three conditions CGD myeloperoxidase deficiency and SAPHO synovitis acne pustulosis hyperostosis and osteitis 2 3 In the given scenario myeloperoxidase deficiency appears more relevant than CGD for the following reasons 1 The disease can be congenital or acquired 3 In this case acquired
Activation of neutrophils with phorbol myristate acetate PMA results in oxidation of DHR to a fluorescent compound rhodamine 123 which can be measured by flow cytometry
Dihydrorhodamine 123 an overview ScienceDirect Topics
Dihydrorhodamine 123 Test
Dihydrorhodamine Test From a Clinical Point of View CHEST
Dihydrorhodamine Flow Cytometric Test Blood Mayo Clinic
The diagnosis of CGD is most easily established by the dihydrorhodamine DHR assay which measures the hydrogen peroxide dependent conversion of DHR 123 to rhodamine 123 which is accompanied by fluorescence
DHR Test for CGD Efficient Testing in CGD Diagnosis For HCPs
Dihydrorhodamine DHR test uses flow cytometry to measure the oxidation of dihydrorhodamine 123 to rhodamine 123 in phorbol myrisate acetate PMA stimulated neutrophils a marker for cellular NADPH oxidase activity Vowells et al 1996 In this test the generation of hydrogen peroxide oxidizes the dye leading to the emission of fluorescence
Dihydrorhodamine 123 flow cytometry method time for
Dihydrorhodamine 123 is a substance used in the diagnosis of Chronic Granulomatous Disease CGD through a specific assay that measures its conversion to rhodamine 123 accompanied by fluorescence to determine superoxide production capacity
Optimized flow cytometry protocol for dihydrorhodamine 123
Dihydrorhodamine oxidation to rhodamine by the respiratory burst of the cell is measured by flow cytometry Results are reported as the ratio of the mean channel fluorescence of stimulated cells versus unstimulated cells which yields a stimulation index SI
Chronic Granulomatous Disease GeneReviews NCBI Bookshelf
Dihydrorhodamine Flow Cytometric Phorbol Test Catalog
INTERPRETATION OF DHR RESULTS FOR CHRONIC GRANULOMATOUS
DHR Flow B is a flow cytometric test that measures NADPH oxidase activity in neutrophils for diagnosis and monitoring of chronic granulomatous disease CGD and other neutrophil disorders The test involves activation of neutrophils with phorbol myristate acetate PMA or N formyl methionyl leucyl phenylalanine fMLP and quantification of dihydrorhodamine DHR fluorescence
The dihydrorhodamine DHR test is the most common test for identifying CGD While some physicians still use the NBT test it has been largely replaced by the flow cytometric DHR test 1 The DHR test has user friendly steps and provides standardized and quantifiable results alongside enhanced sensitivity 1
DHR Overview Dihydrorhodamine Flow Cytometric Test Blood
Benefits of dihydrorhodamine DHR testing The DHR test has the ability to assess neutrophil superoxide production a risk indicator for serious infection Resulting in fewer false negative test results than the NBT test the DHR test is known for its 1 3 Relative ease of use Objectivity without requiring significant operator experience
Your provider may conduct a dihydrorhodamine 123 DHR test or other tests to see how well a type of white blood cell called a neutrophil is functioning Providers usually use this test to diagnose CGD Genetic testing
The dihydrorhodamine 123 assay is generally applied to measure the production of intracellular reactive oxygen species in neutrophils using flow cytometry and is considered a diagnostic evaluation for chronic granulomatous disease
Dihydrorhodamine Flow Cytometric Test Blood Mayo Clinic
Reliability of acute illness dihydrorhodamine 123 testing for
There are several versions of this test in which the dye in the presence of the oxidative burst products is reduced to visibly blue formazan However this test can be insensitive is subjective and laborious
Neutrophil Oxidative Burst Assay DHR ARUP Laboratories
The diagnosis of CGD is most easily established by the dihydrorhodamine DHR assay which measures the hydrogen peroxide dependent conversion of dihydrorhodamine 123 to rhodamine 123 which is accompanied by fluorescence This test is relatively reproducible and can be quantized on a flow cytometer allowing the determination of a DHR index
DHR Testing for Chronic Granulomatous Disease CGD
ICCS eNewsletter Cytometry
DHR assay is the standard diagnostic test for CGD However complete interpretation requires both MFI and percentage of positive cells and variability exists in how DHR is performed and reported by different reference laboratories
Dihydrorhodamine 123 Test
Dihydrorhodamine 123 an overview ScienceDirect Topics
This test evaluates the NADPH oxidase function of neutrophils using dihydrorhodamine as a probe It is used to diagnose chronic granulomatous disease and monitor chimerism and transplantation outcomes
The DHR Flow PMA test measures NADPH oxidase activity in neutrophils using dihydrorhodamine and phorbol myristate acetate It is used to diagnose and monitor chronic granulomatous disease CGD a genetic disorder of phagocyte function
Dihydrorhodamine 123 flow cytometry method time for PubMed
To reach the laboratory within 24 hours and by 2 30pm on a Friday not processed over weekends Store at room temperature Sample type guide
Dihydrohodamine 123 Respiratory Burst Assay DHR